Chromatography

Column packing material comprises of a diol bonded to a high pore volume Ethylene Bridge Hybrid (BEH) substrate. Column is specially QC tested with protein standards.

Column packing material comprises of a diol bonded to a high pore volume Ethylene Bridge Hybrid (BEH) substrate. Column is specially QC tested with protein standards.

Column packing material comprises of a diol bonded to a high pore volume Ethylene Bridge Hybrid (BEH) substrate. Column is specially QC tested with protein standards.

Protein Standards for Chromatography Columns

Quantitation of empty and full AAV capsids is critical to understand and ensure the product quality, safety, and potential efficacy. Multiple techniques have been used in this application however all have some shortcomings and challenges:

Utilizing proprietary surface technologies, Proteomix® HIC-NP resin is made of non-porous polystyrenedivinylbenzene (PS/DVB) beads with narrow-dispersed particle size distribution. As shown in figure below, the PS/DVB bead is modified with alkyl groups or aryl groups that provide hydrophobic interaction with analytes.

They also provide enhanced selectivity and resolution for mAbs, protein fragments, DNA, and oligonucleotides. Built on non-porous PS/DVB beads with a narrow particle size distribution and proprietary alkyl-modified hydrophilic surface technology, these columns provide excellent efficiency, selectivity, and reproducibility. The low back-pressure design also enables high-throughput analysis without compromising separation performance.

Proteomix ion-exchange phases have been innovatively developed for achieving high ion-exchange capacity, a breakthrough technology for non-porous resins. Proteomix packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead with particle size selections of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. The hydrophobic PS/DVB resin surface is totally covered by a hydrophilic coating that eliminates non-specific bindings with biological analytes, leading to high efficiency and high recovery separations for biological molecules. On the top of the hydrophilic layer, ion-exchange functional groups are attached via a proprietary chemistry, resulting in a high capacity ion-exchange layer.

Oligo dT20-mer is conjugated to the bead surface through a proprietary method. The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA). Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Proteomix POR dT20 Affinity Resin surface allows capture of mRNA through base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.