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Monomix dT20 Affinity
Sepax Monomix dT20 Affinity Resin is a narrowly dispersed polymethacrylate rigid base bead, functionalized with a polyhydroxylated surface coating layer that provides a bio-inert surface, which shows low non-specific binding. Oligo dT20-mer is then conjugated to the bead surface through a proprietary method.
Sepax Technologies
Description
The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA). Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Monomix dT20 Affinity Resin surface allows capture of mRNA through base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.
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Features
- Support Matrix: Monomix 1000 Å
- Average Particle Size: 30 µm
- Surface Functionality/Ligand: Oligo dT20
- Binding Capacity: >2.0 mg mRNA per mL of resin
- pH Range: 2-12
- Operating Backpressure/Mechanical Stability: 100 Bar (10 MPa)
- Operating Temperature/Thermal Stability: 4-65°C
- Shipping and Storage Solvent: 20% Ethanol/Water
- Shipping Condition: Wet Ice
Specifications
- Provides efficient capture and release under standard mRNA purification conditions, simplify subsequent purification steps and maximize overall production efficiency.
- Decreases process development time and enhances productivity.
- Allows reduction in plasmid DNA and other transcription mix components.
- Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
- Excellent scalability. Provide prepacked columns, semi-prep column, prep column and bulk resin.
- Non-animal derived
- Resin customization is available. Polymethacrylate bead size is available at 10, 15, 30 and 60µm
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